Methods. GSV was

measured through 3D ultrasound using the planimetric, virtual organ computer-aided analysis (VOCAL) and extended imaging virtual organ computer-aided analysis (XI VOCAL) methods. The planimetric method used a sequence of adjacent planes that are 3 mm thick. For the VOCAL methods, six adjacent planes and a 30 degrees rotation were used. A total of 15 adjacent planes were used for the XI VOCAL method. Regression models with a determination coefficient (R<SU2</SU) were created to assess the correlation between GSV and gestational age (GA). Intraclass correlation coefficient (ICC) and Bland-Altman graphs were used to assess the correlation between the three methods and ANOVA was used to compare means. Results. All AG-881 research buy three methods showed a correlation between GSV and GA (R<SU2</SU = 0.65 for XI VOCAL, R<SU2</SU = 0.65 for planimetric and R<SU2</SU = 0.66 for VOCAL). There was a strong correlation between the three methods (XI VOCAL vs. planimetric ICC = 0.995; XI VOCAL vs. VOCAL ICC = 0.998 and planimetric vs. VOCAL ICC = 0.995) without any significant

differences according to the Bland-Altman graphs or ANOVA Selleck Lonafarnib (p < 0.002). Conclusions. The three 3D ultrasound methods used for GSV assessment between 7 and 11 weeks are concordant. These methods can be used interchangeably during the first trimester of pregnancy to measure GSV.”
“Acute otitis media (AOM) is a common disease of childhood. AOM is most appropriately diagnosed by careful otoscopy with an understanding of clinical signs and symptoms.

The distinction between AOM and chronic otitis media with effusion should be emphasized. Treatment should include pain management, and initial antibiotic treatment should be given to those most likely to benefit, including young children, SU5402 in vivo children with severe symptoms, and those with otorrhea and/or bilateral AOM. Tympanostomy tube placement may be helpful for those who experience frequent episodes of AOM or fail medical therapy. Recent practice guidelines may assist the clinician with such decisions.”
“To determine whether continuous administration of nitrous oxide and remifentanil-either alone or together-alters blood flow in oral tissues during sevoflurane anesthesia. Eight male tracheotomized Japanese white rabbits were anesthetized with sevoflurane under mechanical ventilation. Heart rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP), common carotid arterial blood flow (CCBF), tongue mucosal blood flow (TMBF), mandibular bone marrow blood flow (BBF), masseter muscle blood flow (MBF), upper alveolar tissue blood flow (UBF), and lower alveolar tissue blood flow (LBF) were recorded in the absence of all test agents and after administration of the test agents (50 % nitrous oxide, 0.4 mu g/kg/min remifentanil, and their combination) for 20 min.

The results show that approval rates were similar for rare and common disease applications. Larger company size, prior regulatory experience and priority review designation were BIX 01294 chemical structure associated with higher approval rates. The study findings show that rare disease product development is feasible, and increased interactions between product developers

and FDA in early investigational phases can facilitate product development.”
“Resonance Raman studies have uncovered puzzling complexities in the structures of NO adducts of heme proteins. Although CO adducts of heme proteins obey well-behaved anti-correlations between Fe-C and C-O stretching frequencies, which reflect changes

in backbonding induced by distal H-bonding residues, the corresponding NO data are scattered. This scatter can be traced to distal influences, since protein-free NO-hemes do show well-behaved anti-correlations. Why do distal effects produce irregularities in nu FeN/nu NO plots but not in nu FeC/nu CO plots? We show via density functional theory (DFT) computations on model systems that the response to distal H-bonding differs markedly when the NO acceptor atom is N versus O. Backbonding is augmented by H-bonding to O, but the effect of H-bonding to N is to weaken both N-O and N-Fe bonds. The resulting downward deviation from the nu FeN/nu NO backbonding line increases with increasing H-bond strength. This effect explains the deviations observed for a series of myoglobin variants, in which the strength of distal H-bonding 4EGI-1 is modulated by distal pocket residue 5-Fluoracil manufacturer substitutions. Most of the data follow a positive nu FeN/nu NO correlation with the same slope as that calculated for H-bonding to N. Such deviations are not observed for CO adducts, because the CO pi* orbital is unoccupied, and serves as

a delocalized acceptor of H-bonds. H-bonding to N primes NO-heme for reduction to the HNO adduct, a putative intermediate in NO-reducing enzymes.”
“An 8-year-old male German longhaired pointer was referred for diabetes insipidus responsive to treatment with desmopressin. The dog had polyuria and polydipsia, exercise intolerance and a dull hair coat. Plasma concentrations of thyroid-stimulating hormone, thyroxine, growth hormone (GH) and insulin-like growth factor-I were decreased; plasma adrenocorticotropic hormone (ACTH) was slightly elevated and plasma alpha-melanocyte-stimulating hormone (MSH) was within the reference range. Computed tomography revealed a heterogeneously contrast-enhancing plantar), mass compressing the hypothalamus. Transsphenoidal hypophysectorny was performed and microscopical examination of the surgical biopsy samples revealed hypophysitis without evidence of pituitary adenoma.

The presented tumor was exposed by a growth spurt in approximately 6 months’ time. This manifested in tracheoesophageal compression symptoms (tracheal deviation on roentgenography, significant dyspnea, and dysphagia). Surgical extirpation was recommended as a treatment of choice. Method After positioning patient with rotated head on the contralateral side, a longitudinal incision was made on the anterior border of the sternocleidomastoid GSI-IX muscle extending from the clavicular head to the retromandibular area. The tumor was carefully dissected from structures present in the carotid sheath and fully extirpated

in one piece from its bed, which extended proximally to the collar bone and cranially to the angle of the mandible. A histological examination of the extirpated tumor was performed. Results After the paratracheal tumor was extirpated, an instant relief from the tracheoesophageal compression symptom was described by the patient. Final diagnosis was determined by a histological examination as an ancient schwannoma. Conclusion Currently, the only available treatment for this type of tumor is surgical extirpation. Histological examination is the only method that can establish Selleck Small molecule library final diagnosis.”
“Aromatic-aromatic interactions have long been believed to play key roles in protein structure, folding, and binding functions. However, we still lack

full understanding of the contributions of aromatic-aromatic interactions to protein stability and the timing of their formation during folding. Here, using an aromatic ladder in the beta-barrel protein, cellular retinoic acid-binding protein 1 (CRABP1), as a case study, we find that aromatic pi stacking plays a greater role in the Phe65-Phe71 cross-strand pair, while in another pair, Phe50-Phe65, hydrophobic interactions are dominant. The Phe65-Phe71 pair BTSA1 supplier spans beta-strands 4 and 5 in the beta-barrel, which lack interstrand hydrogen bonding, and we speculate that it compensates energetically for the absence of strand-strand backbone interactions. Using perturbation

analysis, we find that both aromatic-aromatic pairs form after the transition state for folding of CRABP1, thus playing a role in the final stabilization of the beta-sheet rather than in its nucleation as had been earlier proposed. The aromatic interaction between strands 4 and 5 in CRABP1 is highly conserved in the intracellular lipid-binding protein (iLBP) family, and several lines of evidence combine to support a model wherein it acts to maintain barrel structure while allowing the dynamic opening that is necessary for ligand entry. Lastly, we carried out a bioinformatics analysis and found 51 examples of aromatic-aromatic interactions across non-hydrogen-bonded beta-strands outside the iLBPs, arguing for the generality of the role played by this structural motif. (C) 2013 Elsevier Ltd. All rights reserved.

5 GBq (mean, 2.3 GBq; or between www.selleckchem.com/products/ly2090314.html 27 and 121 mCi; mean, 62 mCi) predicated on a prescribed whole-body radiation-absorbed dose of 0.75 Gy were studied. Their 279 family members/carers and 432 visitors wore thermoluminescent dosimeter badges for the week during which the patients were confined to their home after treatment.\n\nResults: All 200 patients received I-131-rituximab activities according to the prescribed dose of 0.75 Gy to the whole body. From 200 consecutive patients, over the 7 days after therapy, mean radiation exposure of adult carers was 0.49 mSv (range, <0.01 to 3.67 mSv). To other coresiding family members, mean exposure

was 0.23 mSv (range, <0.01 to 1.20 mSv), and for visitors sharing badges, the mean exposure was 0.17 mSv (range, <0.01 to 0.73 mSv). Urinary activity excreted over the week after I-131-rituximab

therapy was typically less than MK-2206 manufacturer 25% of the administered activity.\n\nConclusions: I-131-rituximab radioimmunotherapy for non-Hodgkin lymphoma may be safely administered on an outpatient basis. The median radiation exposure of carers, cohabitants of the patient, and visitors is well within the limits recommended by international guidelines. Local regulatory agency-designated patient release rate limit of less than 25 mu Sv/h at 1 m was attained within 1 week of therapeutic I-131-rituximab administration.”
“Objective To investigate the relationship between atopic allergy and depression and the role of DBP in the development of depression.\n\nMethods BALB/c mice were randomly divided HKI272 into eight groups: saline; ovalbumin (OVA)-immunized; saline+DBP (0.45 mg/kgd); saline+DBP (45 mg/kg-c1); DBP (0.45 mg/kgd) OVA-immunized; DBP (45 mg/kg d) OVA-immunized; saline+hydrocortisone (30 mg/kgd); and hydrocortisone (30 mg/kgd)-exposed OVA-immunized. Behavior (e.g. open-field, tail suspension, and forced swimming tests), viscera coefficients (brain and spleen), oxidative damage [e.g. reactive oxygen species (ROS), malondialdehyde (M DA), and glutathione (GSH)], as well as levels of IgE and IL-4, were then analyzed.\n\nResults In the saline and OVA groups, the degree of depression symptoms

in mice increased with increasing DBP concentration. Additionally, the OVA-immunity groups were associated with more serious depressive behavior compared with the same exposure concentration in the saline group. Oxidative damage was associated with a dose-dependent increase in DBP in the different groups. IL-4 and IgE levels were associated with low-dose DBP stimulation, which changed to high-dose inhibition with increasing DBP exposure, possibly due to spleen injury seen at high DBP concentrations.\n\nConclusion Development of an atopic allergy has the potential to increase the risk of depression in mice, and it seems that DBP helps OVA to exert its effect in our present model. Moreover, the results of our study implicate a certain connection between brain oxidative stress and depression, which deserves a further exploration.

Perylene and copper in the sediments could be identified as potential causes of the genotoxic response. To compare the results (maximum induction coefficients) of zebrafish embryos with an established comet protocol, rainbow trout liver cells (RTL-W1) were exposed to the same extracts. The findings correlated well (Spearman correlation r=0.90), proving a good reliability of the results from zebrafish primary cells. In conclusion, the present study demonstrates that the bioavailable fraction of the genotoxic pollutants may pose a threat for both benthic organisms and human health via drinking-water and fish consumption. (C)

2007 Elsevier GW 572016 B.V. All rights reserved.”
“The transcription factor p53 regulates cellular integrity in response to stress. p53 is mutated in more than half of cancerous

cells, with a majority of the mutations localized to the DNA binding domain (DBD). In order to map the structural and dynamical features of the DBD, we carried out multiple copy molecular dynamics simulations (totaling 0.8 mu s). Simulations show the loop 1 to be the most dynamic element among the DNA-contacting loops (loops 1-3). Loop 1 occupies two major conformational states: extended and recessed; the former but not the latter displays correlations in atomic fluctuations with those of loop 2 (similar to 24 angstrom KPT-8602 research buy apart). Since loop 1 binds to the major groove whereas loop 2 binds to the minor groove of DNA, our results begin to provide some insight into the possible mechanism underpinning the cooperative nature of DBD binding to DNA. We propose (1) a novel mechanism underlying the dynamics of loop 1 and the possible tread-milling of p53 on DNA and (2) possible mutations on loop 1 residues to restore the transcriptional activity of an oncogenic mutation at a distant site.”
“A new possibility for estimating the octanol/water coefficient (log P) was investigated using only one descriptor, the semi-empirical electrotopological

index (I(SET)). The predictability of four octanol/water partition coefficient (log P) calculation models was compared using a set of 131 aliphatic organic compounds from five different classes. Log P values were calculated employing LBH589 Epigenetics inhibitor atomic-contribution methods, as in the Ghose/Crippen approach and its later refinement, AlogP; using fragmental methods through the ClogP method; and employing an approach considering the whole molecule using topological indices with the MlogP method. The efficiency and the applicability of the ISET in terms of calculating log P were demonstrated through good statistical quality (r > 0.99; s < 0.18), high internal stability and good predictive ability for an external group of compounds in the same order as the widely used models based on the fragmental method, ClogP, and the atomic contribution method, AlogP, which are among the most used methods of predicting log P.

Design and Participants: A cross-sectional survey of 185 African-Americans admitted to an urban medical center in Maryland, with severe, poorly controlled hypertension from 1999-2004. Categorical and continuous variables were compared using chi-square and t-tests. Adjusted multivariable logistic regression was used to assess correlates of appointment non-adherence. Main Outcome Measures: Appointment non-adherence was the primary outcome and was defined as patient-report of missing greater than 3 appointments out of 10 during their Copanlisib in vitro lifetime. Results: Twenty percent of participants (n = 37) reported missing more than 30% of their appointments. Patient characteristics independently associated with a higher odds of appointment

TH-302 ic50 non-adherence included not finishing high school (Odds ratio [OR] = 3.23 95% confidence interval [CI] (1.33-7.69), hypertension knowledge ([OR] = 1.20 95% CI: 1.01-1.42), lack of insurance ([OR] = 6.02 95% CI: 1.83-19.88), insurance with no medication coverage ([OR] = 5.08 95% CI: 1.05-24.63),

cost of discharge medications ([OR] = 1.20 95% CI: 1.01-1.42), belief that anti-hypertensive medications do not work ([OR] = 3.67 95% CI: 1.16-11.7), experience of side effects ([OR] = 3.63 95% CI: 1.24-10.62), medication non-adherence ([OR] = 11.31 95% CI: 3.87-33.10). Substance abuse was not associated with appointment non-adherence ([OR] = 1.05 95% CI: 0.43-2.57). Conclusions: Appointment non-adherence among African-Americans with poorly controlled hypertension was associated with many markers of inadequate access to healthcare, knowledge, attitudes and beliefs.”
“Brain oxytocin regulates

a variety of social and affiliative behaviors and affects also learning and memory. However, mechanisms of its action at the level of neuronal circuits are not fully understood. The present study tests the hypothesis β-Nicotinamide in vivo that molecular factors required for memory formation and synaptic plasticity, including brain-derived neurotrophic factor, neural growth factor, nestin, microtubule-associated protein 2 (MAP2), and synapsin I, are enhanced by central administration of oxytocin. We also investigated whether oxytocin enhances object recognition and acts as anxiolytic agent. Therefore, male Wistar rats were infused continuously with oxytocin (20 ng/mu l) via an osmotic minipump into the lateral cerebral ventricle for 7 days; controls were infused with vehicle. The object recognition test, open field test, and elevated plus maze test were performed on the sixth, seventh, and eighth days from starting the infusion. No significant effects of oxytocin on anxious-like behavior were observed. The object recognition test showed that oxytocin-treated rats significantly preferred unknown objects. Oxytocin treatment significantly increased gene expression and protein levels of neurotrophins, MAP2, and synapsin I in the hippocampus. No changes were observed in nestin expression.

Exerc. Sport Sci. Rev., Vol. 38, No. 1, pp. 3-9, 2010. Recent studies on the neural bases of sensorimotor adaptation demonstrate that the cerebellar and striatal thalamocortical pathways contribute to early learning. Transfer of learning involves a reduction in the contribution of early learning networks and increased reliance on the cerebellum.

The neural correlates of teaming to team remain to be determined buy A-1331852 but likely involve enhanced functioning of the general aspects of early teaming.”
“Purpose: (1) To determine the current bacteriological spectrum in connatal and acquired lacrimal duct obstruction (cLDO and aLDO, respectively) and (2) to analyze the antimicrobial susceptibility patterns of the recovered isolates. Materials and Methods: In a prospective study, 463 samples (30% bilateral LDO) were obtained from the lacrimal ducts of 132 infants and 192 adult patients with symptomatic LDO between 2007 and 2012 at a tertiary eye-care center. The samples were cultured for aerobic and anaerobic bacteria, which were subsequently identified using

standard microbiological techniques. Antimicrobial susceptibility testing was performed for each isolate using the disk diffusion method. Data were analyzed using SPSS and chi-square test for significance testing. Results: (1) Among 463 samples investigated, 333 samples were positive, i.e. at least one bacterial isolate was recovered. A total of 72% were recovered (97% of samples from children and 56% of samples from adults), yielding a total of 654 bacterial CT99021 datasheet isolates. Co-colonization with up to five different bacterial species MRT67307 in vivo was observed in a large proportion of the samples from children (87%), but in only 20% of those from adults and with a maximum of three different bacteria. Gram-positive bacteria were identified in 72% of the positive samples in both aLDO and cLDO. The most common Gram-positive species in cLDO was Streptococcus pneumoniae (29%), while that in cLDO was Staphylococcus aureus (60%). The most prevalent Gram-negative species were Moraxella catarrhalis (8%) and Haemophilus influenzae (9%) in cLDO and Pseudomonas aeruginosa in aLDO

(12%). (2) Susceptibility testing revealed chloramphenicol to be the most active antibiotic with resistance rates of 3% in cLDO and 6% in aLDO, followed by ciprofloxacin (1% and 6%). Erythromycin and gentamicin were the least active of all, with resistances of 41% and 22%, respectively, in cLDO, and 23% and 11% in aLDO. Conclusions: Bacterial colonization occurs regularly in LDO, with Gram-positive bacteria being found in 97% of cLDO samples and 56% of aLDO samples. A remarkable number of different species were found to co-colonize in cLDO. The most common bacteria in LDO are highly susceptible in vitro to chloramphenicol and ciprofloxacin.”
“Definitive surface markers for retinal progenitor cells (RPCs) are still lacking.

These results suggest that BcMF11, as a non-coding RNA, plays an essential role in pollen development and male fertility.”
“OBJECTIVE To evaluate the role of renal ultrasonography

(USG) in predicting vesicoureteral reflux (VUR) in children with mild renal scarring determined by dimercaptosuccinic acid scintigraphy performed after attack of urinary tract infections (UTI).\n\nMETHODS Dimercaptosuccinic acid scintigraphy, voiding cystourethrography (VCUG), and renal USG findings were evaluated retrospectively in children with UTI. Each kidney was evaluated as a separate renal unit (RU). RUs with severe scarring were excluded from the study. RUs having see more mild scarring with and without abnormal USG findings (group 1 and group 2, respectively) were compared in terms of the presence of

VUR.\n\nRESULTS There were a total of 228 patients (70 men, mean age 47.06 +/- 44.14 months) and 456 RUs. Of the 185 RUs with mild scarring, 55 had abnormal USG findings (group 1), whereas 130 had normal USG findings (group 2). The rates of VUR and severe VUR (>= grade 4) were higher in group 1 compared with group 2 (69% vs 43%, P = .001 and 35% vs 7% respectively, P <.001). The sensitivity, specificity, positive predictive value, negative predictive value, and odds ratio of USG findings selleckchem in predicting VUR in RU with mild scarring were 68%, 80%, 38%, 93%, and 8.2, respectively.\n\nCONCLUSION Normal renal USG findings exclude a diagnosis of high- grade VUR to a large extend in children with UTI and mild renal scarring. Refraining from invasive VCUG might be a reasonable approach in these patients provided that no other predisposing factors for UTI and/or renal scarring present. (C) 2014 Elsevier Inc.”
“This article provides an overview of the current knowledge relating to the potential use of bone marrow-derived mesenchymal stem cells (BM-MSCs)

acting as immunosuppressants after liver transplantation. Clinical use of BM-MSCs in liver transplantation remains experimental, as there is uncertainty as to their mechanism of action, conflicting studies in animal models, and the possibility LY2090314 of their cellular fusion with hepatocytes leading to potentially genetically unstable hepatocytes. These obstacles, to their underuse, have been decreasing, and BM-MSCs have elicited great interest for possible use in solid organ transplantation. Bone marrow-derived-MSCs, when transplanted systemically, might positively influence grafted organ outcome through cell-cell contact or the secretion of soluble factors that are immunomodulatory. Thus, the use of BM-MSCs to modulate organ rejection may directly or indirectly influence the survival properties of transplanted livers. (C) 2012 Elsevier Inc. All rights reserved.

“
“Therapeutic outcome for the treatment of glioma was often limited due to low permeability of delivery systems across the blood brain barrier (BBB) and poor penetration into the tumor tissue. In order to overcome these hurdles, we developed the dual-targeting doxorubicin Liposomes conjugated with cell-penetrating peptide (TAT) and transferrin (T7) (DOX-T7-TAT-LIP) for transporting drugs across the BBB, then targeting brain glioma, and penetrating into the tumor. The

dual-targeting effects were evaluated by both in vitro and in vivo experiments. In vitro cellular find more uptake and three-dimensional tumor spheroid penetration studies demonstrated that the system could not only target endothelial and tumor monolayer cells but

also penetrate tumor to reach the core of the tumor spheroids and inhibit the growth of the tumor spheroids. In vivo imaging further demonstrated that T7-TAT-LIP provided the highest tumor distribution. The median survival time of tumor-bearing mice after administering DOX-T7-TAT-LIP was significantly longer than those of the single-ligand doxorubicin liposomes and free doxorubicin. In conclusion, the dual-ligand liposomes comodified with T7 and TAT possessed strong capability of synergistic targeted delivery of payload into tumor cells both in vitro and in vivo, Mizoribine and they were able to improve the therapeutic efficacy of brain glioma in animals.”
“A multitude of pathophysiologic pathways culminate in the final common denominator of cervical softening, shortening, and dilation that lead to preterm birth. At present, a variety of emerging technology aims to objectively quantify critical cervical parameters such as microstructural organization and softening of the cervix. If the nature and timing of cervical changes can be precisely identified, it should be possible CT99021 concentration to identify the causative upstream molecular processes and resultant biomechanical

events associated with each unique pathway. This would promote molecular studies, ultimately leading to novel approaches to preterm birth prediction, novel treatments, and prevention.”
“1,3-Dipolar cycloaddition of dihydropyran derived intones synthesized from 2,3 dihydro 4H-pyran and various hydroxylamines, with electron deficient alkynes are found to have significant rate acceleration and improved yields of isoxazolines in 1-Butyl-3-methylimidazolium based ionic liquids while with enals exclusively endo isoxazolidines are obtained with high selectivity. Synthetic potentiality of the novel isoxazolines and nitrones have been also tested successfully in peptide and aldehyde synthesis. All the novel isoxazoline and isoxazolidine derivatives have been screened for antimicrobial activities and found to be active.”
“Recent papers indicate that decentralized bioenergy crop production offers increased market access and income diversification strategies for the rural population.

The most promising compounds, etravirine

and rilpivirine, are active on mutant viruses and possess a relatively high genetic barrier for resistance. Data on etravirine resistance in patients already exposed to first-generation non-nucleoside reverse transcriptase inhibitors show that, among 17 mutations in the reverse transcriptase gene, at least three must be present simultaneously in order to diminish etravirine activity. Recent studies of the prevalence of resistance in large databases of patients already exposed to nevirapine and efavirenz show that more than three-quarters of strains will still be sensitive to etravirine in both the southern and northern hemispheres. The first data on rilpivirine resistance are encouraging, but still too preliminary (AIDS Rev. 2009;11:165-73)”
“Objectives: To evaluate periprosthetic von Mises stress distribution with cementless Cilengitide concentration femoral stems of various contours.\n\nMethods: The study was carried out at the Department of Orthopaedics, Shanghai 6th Hospital, Shanghai, China between May 2008 and February 2009. Salubrinal mw Finite element models of proximal femoral replacement

with 4 cementless stems (Alloclassic, Ribbed Anatomic, VerSys, and Securi-fit) of various contours were set up. Under the loading conditions of walking and stair climbing, 3-dimensional periprosthetic von Mises stresses were calculated, and the stress distribution patterns were compared.\n\nResults: Periprosthetic stresses were increased in level 1, 2, and 3 under the 2 loading conditions, and more

considerably in level 2 and 3. The stresses were higher on the medial side in all cases. No remarkable difference was found in the patterns between the 4 stems.\n\nConclusion: find more The contour design of femoral stem has minor effect on initial periprosthetic von Mises stress distribution.”
“Purpose: To determine whether a dynamic cultured biograft can positively affect the function of the damaged heart.\n\nMethods: We ligated the coronary artery (LAD) of rats to generate a model of myocardial infarction (MI) and then implanted them with the following grafts comprising vascular smooth muscle cells (VSMCs) derived from the rat aorta and seeded onto biodegradable patches (patch replacement therapy; (PRTx)): control without PRTx, PRTx without seeded cells, PRTx with static cultured VSMCs, PRTx with dynamic cultured VSMCs and sham-operated. Cultured VSMCs were labeled with PKH26 for identification after implantation, and the centre of the MI site was excised and replaced with an implanted biograft. Cardiac performance was monitored for 12 weeks thereafter and followed by a histological study.\n\nResults: Although the ejection fraction of the damaged heart improved in all groups that were transplanted with grafts, remodeling was prevented only in groups with a dynamic or static cultured patch. More cells were alpha-SMA-positive in the group with the dynamic, rather than the static cultured patch.