Such recombination processes may significantly influence bacterial diversity (Kobayashi, 2001). R-M systems can also be considered as mobile elements, as suggested by their amplification, mobility, and involvement in genome rearrangements, as well as their mutual competition and regulation of gene expression (Ishikawa et al., 2010). Type II R-M systems are usually located in bacterial

and archaeal chromosomes, although they are sometimes found in plasmids, which may disseminate GSK J4 manufacturer these systems among diverse bacterial populations. In a few cases, R-M modules may play an important role in the biology of bacterial plasmids, since they are able to stabilize these replicons in a bacterial population by eliminating plasmid-less cells at the postsegregational level (e.g. Kulakauskas et al., 1995). The vast majority of plasmid-encoded type II R-M systems have been identified LY294002 ic50 in (1) Enterobacteriaceae (e.g. Klebsiella pneumonia RFL2; Lubys et al., 1999) and (2) lactic acid bacteria (e.g. Lactococcus lactis W56; Kong & Josephson, 2002). Much less is known about the R-M systems of other groups of bacteria. For example,

to our knowledge, only one plasmid-encoded R-M module has been described in the Alphaproteobacteria, whose genomes are known for their multi-replicon structures (Rochepeau et al., 1997). Recently we have performed complex genomic studies of a pool of 17 plasmids residing in bacteria of the genus Paracoccus (Alphaproteobacteria). Detailed analysis of the obtained nucleotide sequences revealed that one of the plasmids (pAMI7 of Paracoccus aminophilus JCM 7686) contains a type II R-M system (Dziewit et al., 2011). In this study we present a molecular and functional characterization of the components of this system. The following bacterial strains were used in this study:

(1) P. aminophilus JCM 7686 (Urakami et al., 1990), (2) Paracoccus pantotrophus KL100 (Bartosik et al., 2002), and (3) Escherichia coli TG1, TOP10 and MC1000 (Casadaban & Cohen, 1980). All strains were grown in lysogeny broth medium (Sambrook & Russell, 2001) at 37 °C (E. coli) or 30 °C (Paracoccus spp.). Where necessary, Alectinib molecular weight the medium was supplemented with antibiotics at the following concentrations: ampicillin – 100 μg mL−1, kanamycin – 50 μg mL−1, rifampicin – 50 μg mL−1, and tetracycline – 20 μg mL−1. The plasmids used in this study are listed in Table 1. The nucleotide sequence of pAMI7 was analyzed using Clone Manager (Sci-Ed8) and artemis software (Carver et al., 2008). Similarity searches were performed using the blast programs (Altschul et al., 1997) provided by the NCBI (http://blast.ncbi.nlm.nih.gov/Blast.cgi) and REBASE (http://rebase.neb.com/rebase/rebase.html). The restriction and modification activity of E.