Biological activity was demonstrated using an Agrobacterium tumefaciens indicator strain. Secondly, when added to R. rubrum cultures, their effect was to reproduce and strengthen the responses of PM production and growth rates. selleck screening library In the related species Rhodobacter sphaeroides, a single AHL (7,8-cis-N-(tetradecenoyl)-HSL) has been reported so far, apparently associated with polysaccharide formation and cell aggregation [12]. However, to our knowledge, the present study is the first report showing that AHL autoinducer molecules correlate with photosynthetic gene expression in anoxygenic photosynthetic bacteria and the first profiling of AHLs at different

growth modes in these bacteria. In particular, the extreme heterogeneity in the abundance of the individual molecular species in phototrophic vs. chemotrophic grown cells suggest that these compounds contribute to the versatile

physiological adaptation of this organism to changing light and oxygen conditions. In particular, the appearance of C8OH-HSL at later stages of Fed-Batch cultivations and general correlation with PM repression in microaerobic cultures, in combination with the respective effect when the purified compound is applied to R. rubrum, makes C8OH-HSL a major candidate as a signaling molecule involved in PM formation under microaerobic conditions. We cannot exclude at present that the six AHLs identified in this study do not reflect the complete repertoire of AHLs synthesized click here by R. rubrum. The employed HPLC Selleckchem JNJ-64619178 elution

profile might have missed for example EPZ015938 mw low chain length (C4-HSLs) and/or long chain (C14-HSL) compounds as well as AHLs of very low abundance. Based on our results, C6OH-HSL during phototropic growth with fully expressed PM, and C8OH-HLS in microaerobic chemotrophic cells with inhibited PM expression appear to be major complementary players in the contribution of quorum sensing to photosynthetic gene expression. Moreover the results of the present study suggest that AHL levels can significantly influence growth rates. It has been reported that bacteria with acyl-HSL-degrading activity can grow on a basal growth medium containing 3-oxo-hexanoyl-L-HSL as the sole carbon and nitrogen source [29, 30]. As R. rubrum possesses homologues of AHL degrading proteins (PvdQ and AiiA homologues, see Additional file 1: Table S2), we expected the enhanced growth to be related to an additional supply of carbon source. However, as higher AHL amounts seem to suppress the initial cell growth the observations of Chan et al.[30] and Leadbretter et al.[29] seems to be inadequate to explain the observed behavior. Therefore, these results suggest a non-nutritional role for AHLs in their effect on growth rates. Effect of bacteriochlorophyll a precursor on PM synthesis During the previous development of HCD Fed-Batch cultivation for R.