Nociceptive behavior (mechanical allodynia and weight-bearing distribution of hind paws) during OA development was analyzed pre- and 3, 6, 9, 12, 15, and 18 weeks post-ACLT. Macroscopic and histologic studies were then performed on the cartilage and synovia. Immunohistochemical analysis was performed to examine the effect of glucosamine on expression of mitogen-activated protein kinases (MAPKs) in the articular cartilage chondrocytes.
Results: selleckchem OA rats receiving glucosamine showed a significantly lower degree of cartilage degeneration than the rats
receiving placebo. Glucosamine treatment also suppressed synovitis. Mechanical allodynia and weight-bearing distribution studies showed significant improvement in the OA + glucosamine group as compared to the OA group. Moreover, glucosamine attenuated p38 and c-Jun N-terminal kinase (JNK) but increased extracellular signal-regulated kinase 1/2 (ERK) expression in OA-affected cartilage.
Conclusion: Our results indicate that treatment with oral glucosamine sulfate in a rat OA model (1) attenuates the development of OA, (2) concomitantly reduces nociception, and (3) modulates chondrocyte metabolism, possibly through selleck chemical inhibition of cell p38 and JNK and increase of ERK expression. (C) 2010 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.”
“Osteomyelitis is an acute or
chronic inflammatory process that occurs in bone. Although cranial bones are rarely involved, timely diagnosis and early treatment are mandatory because of its proximity to critical neurovascular structures and aesthetic considerations. A 57-year-old
woman, who had a comminuted open nasal bone fracture 20 years ago, presented a firm, painful swelling nose. Whole-body bone scan images indicated increased uptake of the nasal bone, which was suspected to be osteomyelitis. After debridement, the nose was reconstructed with a thin latissimus dorsi perforator flap. In this article, we report a rare case of chronic osteomyelitis of the nose and provide a brief review of the literature. It is important for clinicians to consider osteomyelitis for early diagnosis and treatment.”
To compare the dexamethasone concentration in various structures of the feline eye following a single topical application of dexamethasone as an ophthalmic learn more ointment or suspension.
Nineteen cats, euthanized due to reasons not related to this study, were selected and their ocular health status evaluated. Selected animals were treated with dexamethasone ointment or suspension.
The concentration of dexamethasone was determined in the following structures of the eye: third eyelid, cornea, aqueous humor, iris, lens, vitreous body, and choroid/retina. The dexamethasone concentration in the eye was measured by radioimmunoassay. The applied amount of dexamethasone was 0.05 mg in 0.05 mL Isopto Dex (R) ophthalmic suspension and 0.