​(Fig4C4C and Fig 6D) and/or the caudate-putamen nucleus or the

​(Fig.4C4C and Fig. 6D) and/or the caudate-putamen nucleus or the hippocampus (see below), depending on the position of their perikaryon in the cc. Moreover, many NADPH-d+/NOSIP located over the lateral ventricle sent dendrites as far as the ependymal layer. Figure 6 Morphology of NADPH-d+ neurons in the rat corpus callosum. (A) A bipolar NADPH-d+ intracallosal neuron with long dendrites extending along the VE-822 purchase rostrocaudal axis of the corpus callosum. (B) A pyriform NADPH-d+ neuron in the ependymal region. (C) Three … Some NADPH-d+/NOSIP neurons located in layer VI of the cerebral cortex, the white matter, or the caudate-putamen nucleus

had dendrites reaching Inhibitors,research,lifescience,medical the cc. Bundles Inhibitors,research,lifescience,medical of labeled beaded processes that were not

in continuity with neighboring NADPH-d+/NOSIP neurons could be observed along the rostrocaudal extension of the cc. Several labeled neurons were also seen around the ependymal layer of the lateral ventricle (Fig. ​(Fig.5A5A and E, Fig. ​Fig.6B6B and E). Figure 5 Photomicrographs of nNOSIP neurons in the rat corpus callosum. (A) Inhibitors,research,lifescience,medical Low-power photomicrograph showing the distribution of nNOSIP neurons. (B) A bipolar neuron close to an intracallosal blood vessel. Framed area enlarged in C. (C) Enlarged area showing … Neurons positive for NADPH-dHi were counted in two brains (CC-NADPH-10, -11, both hemispheres; see Table ​Table2),2), carefully avoiding including labeled neurons from the overlying white matter or the dorsal hippocampal commissure. In CC-NADPH-10, neurons were counted in 145 50-μm thick sections (accounting overall for 7250 μm of thickness); in CC-NADPH-11, sections were 130 and their thickness was 50 μm (overall Inhibitors,research,lifescience,medical 6500 μm of thickness). In CC-NADPH-10,

there were 2959 positive neurons (on average 20.4 neurons/section); Inhibitors,research,lifescience,medical of these, 2583 lay in the cc body and 376 (12.70%) in the ependymal region of the cc; in CC-NADPH-11, there were 2227 NADPH-d+ neurons (on average 17.1/section) of which 2029 were located in the body and 198 (8.89%) in the ependymal region. Ependymal from neurons had a predominantly fusiform morphology. Counts performed in 278 pooled neurons from cases CC-NADPH-5, -7, -9 indicated that 46.76% (130/278) were fusiform, 25.17% (70/278) were polygonal, and that round and pyramidal neurons accounted for 19.06% (53/278) and 8.99% (25/278), respectively. Morphology of NADPH-d+ neurons All NADPH-d+ neurons found in the cc were intensely stained and showed a Golgi-like appearance. Labeled neurons allowed studying the morphology of cc neurons whose somatic and dendritic characteristics enabled their classification into five distinct types: bipolar (fusiform, rectangular), round, polygonal (quadrangular), and pyramidal (triangular-pyriform). Bipolar neurons These neurons were about 28.03% (see Table ​Table3)3) of the entire population of NADPH-d+ intracallosal neurons.

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